HUMANIN

• High‑Purity Synthesis: Solid‑phase synthesized and purified by HPLC (≥98%), with sequence accuracy confirmed by mass spectrometry
• Reliable Bioactivity: Validated anti‑apoptotic and cytoprotective activity in multiple cell lines (e.g., neuronal cells, endothelial cells)
• Stable Formulation: Lyophilized powder ensures long‑term storage and shipping stability; reconstituted solution maintains consistent activity for in vitro and animal studies
• Comprehensive QC: Certificate of Analysis provided, including purity, content, and sterility data

Humanin exerts protection through multi‑target, multi‑pathway synergy. Key mechanisms include:

1. Binding Specific Receptors: Interacts with cell‑surface receptors (e.g., CNTFR/WSX‑1/gp130 tripartite complex, Formyl Peptide Receptor 2), activating downstream pro‑survival pathways such as STAT3 and Akt
2. Direct Mitochondrial Regulation: Localizes to mitochondria, inhibiting mitochondrial‑dependent apoptosis (e.g., reducing cytochrome c release, suppressing Bax translocation)
3. Inhibition of Neurotoxic Signaling: Attenuates toxicity induced by Aβ, mutant APP, or huntingtin proteins in neurodegenerative disease models
4. Metabolic Modulation: Improves insulin sensitivity, reduces oxidative stress and inflammatory responses in models of metabolic disorder

• Reconstitution: Reconstitute with sterile saline or phosphate‑buffered saline (PBS). Gently vortex to dissolve; avoid vigorous shaking or repeated freeze‑thaw cycles
• In Vitro Cell Experiments:Working Concentration: Typical range 0.1–10 μM; optimal concentration should be determined by pilot experimentsTreatment Duration: From several hours for acute protection to 24–72 hours for long‑term effects
• Animal Studies:Administration Routes: Intraperitoneal, intravenous, or local delivery (e.g., intracerebroventricular)Dosage Suggestions: Common dose range 0.5–5 mg/kg/day; optimize based on model and duration
• Storage:Lyophilized Powder: Store at ≤ ‑20°C, protected from light, for optimal long‑term stabilityReconstituted Solution: Aliquot immediately and store at ‑80°C; avoid repeated freeze‑thaw cycles (single‑use aliquots recommended)
• Critical Notes:Research Use Only: Not for human or clinical useActivity Validation: Include positive controls (e.g., known apoptosis inducers) and negative controls (no‑peptide treatment) in key experimentsEthical Compliance: Animal studies must adhere to institutional ethical review and animal welfare guidelines

Packaging Note: This product is supplied in a sealed, sterile glass vial without any pre‑printed labels. This design supports institutional compliance protocols and allows researchers to apply custom labels that align with specific experimental protocols, ethical approvals, and local regulations. A separate document containing complete product details (name, batch number, purity, content, COA reference, etc.) is included for label customization and record‑keeping.