SLU-PP-332

• High MC4R Selectivity: Exhibits high affinity and selectivity for MC4R, suppressing its constitutive activity or agonist-induced activation, suitable for inverse agonism/antagonism studies
• Optimized Stability & Permeability: Structurally modified to enhance metabolic stability; some derivatives may possess blood-brain barrier penetration potential for central and peripheral metabolic research
• Rigorous QC Validation: Solid-phase synthesized and purified via HPLC (≥98%), with both mass spectrometry (MS) and functional activity validation (e.g., cAMP assays)
• Multi-Model Applicability: Suitable for cell models (MC4R-overexpressing cell lines), brain slices, and in vivo metabolic studies

• Reconstitution: Prepare a high-concentration stock solution (e.g., 10-50 mM) in DMSO, then dilute to working concentration with culture medium or saline. Avoid freeze-thaw cycles of the stock solution
• In Vitro Experiments:Typical Concentration: Working concentrations range from 0.1–10 μM in cell experiments; optimize based on cell type and MC4R expression levelsTreatment Duration: Short-term signaling assays (e.g., cAMP) can be performed within minutes; gene expression or metabolic phenotype assays may require 24-72 hours
• Animal Studies:Administration Routes: Intracranial (intracerebroventricular or specific nuclei), intraperitoneal, or subcutaneous injectionDosage Suggestions: Common doses in animal research range from 1–10 mg/kg; optimize based on model and observed endpoints
• Storage:Lyophilized Powder: Store at ≤ -20°C, protected from lightStock Solution: Aliquot and store at -80°C for long-term stability; avoid repeated freeze-thaw cycles
• Critical Notes:Research Use Only: Not for human or clinical useSolvent Control: When using DMSO, keep final concentrations ≤ 0.1% (cells) or ≤ 5% (animals), and include solvent control groupsMechanistic Validation: Functional validation with MC4R agonists (e.g., α-MSH) is recommended, and effects should be confirmed via cAMP assays or relevant downstream markersEthical Compliance: Animal studies must adhere to institutional ethical review and animal welfare guidelines

• Reconstitution: Reconstitute with a sterile pH-buffered solution (e.g., 0.1M NaOH or phosphate buffer adjusted to pH 8.0–9.0) to maintain stability and solubility
• In Vitro Experiments:Typical concentration range for receptor activation assays: 0.1 nM – 100 nMPre-experimental optimization recommended for cAMP or insulin secretion assays
• Animal Studies:Dosage: Common doses in mouse/rat studies range from 0.1–1 mg/kg, administered via subcutaneous injection once or twice weeklyModel Applications: Suitable for obesity, type 2 diabetes, non-alcoholic fatty liver disease, and other metabolic disorder models
• Storage:Lyophilized Powder: Store at ≤ -20°C protected from light; long-term storage at -80°C is recommendedReconstituted Solution: Aliquot immediately and store at -80°C; avoid repeated freeze-thaw cycles (single-use aliquots suggested)
• Critical Notes:Research Use Only: This product is for research purposes only and is not intended for human or clinical useMechanistic Validation: Effects should be validated using receptor activation assays, insulin/glucagon measurements, glucose tolerance tests, etc.Ethical Compliance: Animal studies must be approved by institutional animal ethics committees and follow animal welfare guidelines

Packaging Note: This product is supplied in a sealed, sterile glass vial without any pre‑printed labels. This design supports institutional compliance protocols and allows researchers to apply custom labels that align with specific experimental protocols, ethical approvals, and local regulations. A separate document containing complete product details (name, batch number, purity, content, COA reference, etc.) is included for label customization and record‑keeping.